Total-Transcriptome cDNA Synthesis Kit

Beschreibung

A complete RNA quantification by revealing all the different RNAs in a sample. Crb Total-Transcriptome cDNA Synthesis kit provides all reagents necessary to generate high quality cDNA from all different kind of RNA in a sample such as mRNA, miRNA, and lncRNA. This is made possible by 3' poly(A)-tailing of RNAs via the use of poly(A) polymerase. Oligo d(T) adaptor and random primers then anneal onto the RNA template and facilitate the reverse transcription. OneScript Plus Reverse Transcriptase is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The OneScript Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 55°C); facilitating the elimination of secondary structures associated with GC-rich RNA templates. The recombinant RNaseOFF Ribonuclease Inhibitor effectively protects RNA templates from degradation. Also the universal 3' reverse primer included in the kit can be coupled with any specific forward primer in downstream qPCR/PCR amplification Key Features Revealing all the RNAs in a human or mouse RNA sample A complete, comprehensive cDNA synthesis kit Including oligo dT adaptor and Universal reverse primer for high efficiency Including positive control assays for mRNA, miRNA, and lncRNA studies Robust cDNA synthesis from any RNA template High reproducibility and excellent yield