10×PCR Buffer 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4, 15mM MgCl2, 0.5% NP-40
Unit definition One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions.
Applications • PCR amplification of DNA fragments up to 5 kb • DNA labeling and DNA sequencing • PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR
Taq DNA Polymerase is a highly thermostable DNA polymerase of a thermophilic bacterium Thermus aquaticus. Taq DNA Polymerase catalyzes 5'=>3' synthesis of DNA. The enzyme has no detectable 3'=>5' proofreading exonuclease activity and possesses low 5'=>3' exonuclease activity. This enzyme adds a single 3'-A overhang to each end of the PCR product, which can be applied to T/A cloning. The recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.
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